Ip injection pregnant mouse

IP injection on pregnant mice ... Recently I need to ip injection to pregnant mice in GD 10 - GD 17. however, our lab haven't do it before and I am afraid the needle will get into the uterus and ...

Recently I need to ip injection to pregnant mice in GD 10 - GD 17. however, our lab haven't do it before and I am afraid the needle will get into the uterus and hurt the fetus.

5. Inject pregnant female mouse using 1 ml syringe (BD 309602) and 21G 5/8 needle (BD 305122) in the animal room, and inject between inner thigh and genitals for IP injections to ensure no organs are punctured. 6. Inject slowly and leave needle in around 2 seconds to minimize back flow or …

If any swelling at the injection site or resistance to injection occurs, remove the needle and reinsert it slightly above the initial injection site. Intradermal Injections In order to perform intradermal injections, the mouse should be anesthetized. Shave or pluck an injection site …

Injection Intramuscular Injection: Intraperitoneal Injection : Intravenous Injection Subcutaneous Injection Mouse. Skin Volume: <0.05ml Needle: <25 Gauge Rear Leg/Thigh Volume: <0.05ml Needle: <23 Gauge ... Injection Routes, Maximum Needle Sizes & Volumes; Author: NIH Created Date:

A video demonstration of IP injection technique. Skip to content. | Skip to navigation. Search People ... Central Tail Artery Blood Collection in the Mouse. Oral Gavage in Mice. Intraperitoneal Injection in Mice. Retro-orbital Injection in the Mouse.

Lift a mouse by the base of the tail and place it on the cage lid, wire bar cage top, or a similar rough surface. One handed mouse restraint is usually performed with the non-dominant hand, leaving the dominant hand free for use. An alternative method allows the technician to use their lab coat or ...

Recommended Dose Volumes for Common Laboratory Animals IQ 3Rs Leadership Group - Contract Research Organization Working Group nor does it recommend intraperitoneal dosing in pregnant rodents. Intramuscular dosing in rats, mice or hamsters is not recommended unless there are exceptional circumstances. (p.18).

MOUSE BIOMETHODOLOGY Marcel I. Perret-Gentil, DVM, MS University Veterinarian & Director Laboratory Animal Resources Center The University of Texas at San Antonio (210) 458-6173 larc@utsa.edu PURPOSE OF THIS DOCUMENT This is a handout that accompanies a hands-on mouse biomethodology workshop in the Laboratory Animal Resources Center (LARC).

One of the uses of tamoxifen in the lab is the activation of enzymes deactivated via an estrogen-binding domain, like Cre-ER T (Cre-LBD). Tamoxifen is an estrogen analogue that binds with higher affinity than estrogen to altered estrogen binding domains. There are several ways to administer tamoxifen to mice each with advantages and disadvantages.

Use a 27-gauge needle and inject the mouse with 9mg/40gr body weight, 3x times, one every other day (you can do up to 5 injections continuously) Determine recombination one week after the last TM injection; For pregnant females: Inject once with 3mg/40gr body weight. C. Section embryos and transfer them to a foster mother

3/10/2010 · In one experiment, one control mouse and one running mouse received a single intraperitoneal injection of EdU (200 mg/kg) and a single injection of BrdU (243.5 mg/kg). In the other experiment, two control mice were treated in the same way. Mouse brains were harvested 4 hours or 30 days after injection of the thymidine analogue.

For IP injection of Doxycycline: 10 mcg of Doxycycline in 0.5 cc of water can be given as a single IP injection to a 30 gram mouse. This is only necessary for very sick mice. For administration of Doxycycline in food pellets: Doxycycline in food pellets are custom made by Bio-Serv (908-996-2155) One 8th Street, Frenchtown, NJ 08825.

8/7/2006 · To estimate the duration of effective tamoxifen levels after IP injection, tamoxifen was administered at different times and embryos were allowed to develop until E13.5 before analysis to assess how early a single dose can be given and still achieve efficient Cre activation and recombination within a given, forming P‐D limb component (Fig. 4).

Start studying Lab Animal Care (Lectures 1-4, AALAS 1-2). Learn vocabulary, terms, and more with flashcards, games, and other study tools. ... In the mouse, IP injection should proceed only after aspiration has shown that the needle is properly positioned. ... There is a risk of transmission to the fetus of pregnant women exposed to LCM. It is ...

5/20/2018 · Recent outbreaks of infectious neuro-developmental diseases such as congenital Zika syndrome - have led to a demand for prognosis data from animal models. We developed an intra-amniotic injection mice model that allows Zika virus (ZIKV) infected mice to grow to puberty. In this system, ZIKV is injected into the amniotic fluid of pregnant mice and infected embryos thereafter.